Bactericidal Effects of High Airborne Ozone Concentrations
on Escherichia coli and Staphylococcus aureus

by W. J. Kowalski1, W. P. Bahnfleth1, and T. S. Whittam2


The Pennsylvania State University
Department of Architectural Engineering
2Department of Biology
University Park, PA 16802


 The bactericidal effects of high concentrations of airborne ozone were tested against Escherichia coli and Staphylococcus aureus. Petri dishes containing the microorganisms were inserted in a chamber and exposed for 10 - 480 seconds to ozone concentrations between 300 and 1500 ppm. Death rates in excess of 99.99% were achieved for both species. The mechanism of inactivation accorded with the predictions of first and second-order kinetics, suggesting that the disinfection action of ozone in air parallels the action of ozone in water.

Ozone, Disinfection, Airborne Pathogens, Respiratory Infections, Microbial IAQ, Ultrasonication, Disinfection Kinetics, Survival Curves, Tailing, Clumping, E. coli, S. aureus, Surface Disinfection.